SSC Buffer (20 X)

SSC Buffer (20X) is a concentrated solution of sodium chloride-sodium citrate in distilled/deionized water. SSC Buffer is widely used in nucleic acid blotting and hybridization protocols. SSC Buffer (20X) is already in solution, which eliminates the weighing, mixing, and adjusting of pH necessary with powdered buffers. The 20X formulation allows for easy dilutions to all needed concentrations. SSC Buffer (20X) contains 3M sodium chloride and 0.3M sodium citrate (pH 7.0).